Micropropagation of Plumbago auriculata Plants by Nodal Segment Explants

El-Wakeel, Soad A.; Abdel-Maksoud, Bassiouny A.; El-Shennawy, Ola A.; El-Shanhorey, Nader; Aboelhasan, Fatma M. O.

doi:10.21608/alexja.2025.353827.1122

Micropropagation of Plumbago auriculata Plants by Nodal Segment Explants

Document Type : Original Article

Authors

¹ Department of Botanical Gardens Research -Antoniades, Horticultural Research Institute, Agriculture Research Center, Alexandria, Egypt

² Department of Floriculture, Ornamental Horticulture and Landscape Design- Faculty of Agriculture, Alexandria University, Alexandria, Egypt

³ Maryout Research Station, Genetic Resources Department- Desert Research Center, Alexandria, Egypt

10.21608/alexja.2025.353827.1122

Abstract

The study was conducted during the period from 2022 to 2024 at the Tissue Culture laboratory at the Department of Floriculture, Ornamental Horticulture and Landscape Design - Faculty of Agriculture, Alexandria University and Maryout Research station, Genetic Resources Department, Desert Research Center, Egypt where Plumbago auriculata Lam was micropropagated. The study aimed to develop a protocol for tissue culture propagation of Plumbago. Nodal segments were taken and cultured on MS medium in the presence of cytokinin (BA) at different concentrations: 0.0, 0.5, 1.0, 1.5, and 2.0 mg/L, in conjunction with auxin (IAA) at different concentrations: 0.0, 0.5, 1.0 and 1.5 mg/L for 30 days. A concentration of 1.5 mg/L of IAA with a concentration of 1.00 mg/L of BA resulted in the highest number of shoots and the longest shoot length. After the vegetative shoots reached a length of 5 cm, they were transferred to a rooting medium containing of plant growth regulators (IAA and IBA) concentration of 0.01 mg/l for 4 weeks, achieving a rooting percentage of 95%. Then the plants were transferred and planted in pots containing peat moss and sand in a 1:1 ratio, and the acclimatization process was successfully completed with a 95%.

Keywords

Main Subjects