El-Wakeel, S., Abdel-Maksoud, B., El-Shennawy, O., El-Shanhorey, N., Aboelhasan, F. (2025). Micropropagation of Plumbago auriculata Plants by Nodal Segment Explants. Alexandria Journal of Agricultural Sciences, 70(2), 162-170. doi: 10.21608/alexja.2025.353827.1122
Soad A. El-Wakeel; Bassiouny A. Abdel-Maksoud; Ola A. El-Shennawy; Nader El-Shanhorey; Fatma M. O. Aboelhasan. "Micropropagation of Plumbago auriculata Plants by Nodal Segment Explants". Alexandria Journal of Agricultural Sciences, 70, 2, 2025, 162-170. doi: 10.21608/alexja.2025.353827.1122
El-Wakeel, S., Abdel-Maksoud, B., El-Shennawy, O., El-Shanhorey, N., Aboelhasan, F. (2025). 'Micropropagation of Plumbago auriculata Plants by Nodal Segment Explants', Alexandria Journal of Agricultural Sciences, 70(2), pp. 162-170. doi: 10.21608/alexja.2025.353827.1122
El-Wakeel, S., Abdel-Maksoud, B., El-Shennawy, O., El-Shanhorey, N., Aboelhasan, F. Micropropagation of Plumbago auriculata Plants by Nodal Segment Explants. Alexandria Journal of Agricultural Sciences, 2025; 70(2): 162-170. doi: 10.21608/alexja.2025.353827.1122
Micropropagation of Plumbago auriculata Plants by Nodal Segment Explants
1Department of Botanical Gardens Research -Antoniades, Horticultural Research Institute, Agriculture Research Center, Alexandria, Egypt
2Department of Floriculture, Ornamental Horticulture and Landscape Design- Faculty of Agriculture, Alexandria University, Alexandria, Egypt
3Maryout Research Station, Genetic Resources Department- Desert Research Center, Alexandria, Egypt
Receive Date: 25 January 2025,
Revise Date: 27 February 2025,
Accept Date: 27 February 2025
Abstract
The study was conducted during the period from 2022 to 2024 at the Tissue Culture laboratory at the Department of Floriculture, Ornamental Horticulture and Landscape Design - Faculty of Agriculture, Alexandria University and Maryout Research station, Genetic Resources Department, Desert Research Center, Egypt where Plumbago auriculata Lam was micropropagated. The study aimed to develop a protocol for tissue culture propagation of Plumbago. Nodal segments were taken and cultured on MS medium in the presence of cytokinin (BA) at different concentrations: 0.0, 0.5, 1.0, 1.5, and 2.0 mg/L, in conjunction with auxin (IAA) at different concentrations: 0.0, 0.5, 1.0 and 1.5 mg/L for 30 days. A concentration of 1.5 mg/L of IAA with a concentration of 1.00 mg/L of BA resulted in the highest number of shoots and the longest shoot length. After the vegetative shoots reached a length of 5 cm, they were transferred to a rooting medium containing of plant growth regulators (IAA and IBA) concentration of 0.01 mg/l for 4 weeks, achieving a rooting percentage of 95%. Then the plants were transferred and planted in pots containing peat moss and sand in a 1:1 ratio, and the acclimatization process was successfully completed with a 95%.
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