EL-Hefny, M., Abd El-Kareem, M., Salem, M. (2022). GC-MS and HPLC analyses of phytochemical compounds from Withania somnifera L. leaves extract. Alexandria Journal of Agricultural Sciences, 67(1), 10-17. doi: 10.21608/alexja.2022.131511.1016
Mervat EL-Hefny; Mamoun S.M. Abd El-Kareem; Mohamed Z.M. Salem. "GC-MS and HPLC analyses of phytochemical compounds from Withania somnifera L. leaves extract". Alexandria Journal of Agricultural Sciences, 67, 1, 2022, 10-17. doi: 10.21608/alexja.2022.131511.1016
EL-Hefny, M., Abd El-Kareem, M., Salem, M. (2022). 'GC-MS and HPLC analyses of phytochemical compounds from Withania somnifera L. leaves extract', Alexandria Journal of Agricultural Sciences, 67(1), pp. 10-17. doi: 10.21608/alexja.2022.131511.1016
EL-Hefny, M., Abd El-Kareem, M., Salem, M. GC-MS and HPLC analyses of phytochemical compounds from Withania somnifera L. leaves extract. Alexandria Journal of Agricultural Sciences, 2022; 67(1): 10-17. doi: 10.21608/alexja.2022.131511.1016
GC-MS and HPLC analyses of phytochemical compounds from Withania somnifera L. leaves extract
1Department of Floriculture, Ornamental Horticulture and Garden Design, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt
2Atomic and Molecular Physics Unit, Experimental Nuclear Physics Department, Nuclear Research Centre, Egyptian Atomic Energy Authority, Inshas, Cairo, Egypt
3Forestry and Wood Technology Department, Faculty of Agriculture (El–Shatby), Alexandria University, Egypt
Receive Date: 04 March 2022,
Revise Date: 11 March 2022,
Accept Date: 14 March 2022
Abstract
In the present work, the shrub W. somnifera was used as a source for the extraction of bioactive molecules. Leaves were extracted by ethanol and analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) and High-Performance Liquid Chromatography (HPLC). By GC-MS analysis, the highest identified compounds were 2,6-dimethyl-N-(2-methyl-α-phenylbenzyl)aniline, 4-Aminoheptane, n-undecanophenone, 2-cyclohexyl-4-hydroxymethyl-octahydrobenzo[e][1,2] oxazine-3-carbonitrile, and octadecanoic acid 3-octadecyloxypropyl ester, with percentages of 19.43%, 7.51%, 4.47%, 4.34%, and 3.89%, respectively. HPLC analysis identified quercetin and 7-hydroxyflavone with concentrations of 12.36 and 15.14 mg/mL, respectively, as two flavonoid compounds. This work showed that W. somnifera leaf extract owns several bioactive compounds that could be useful and have different applications, such as antimicrobial activities.
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